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JoVE Journal
Neuroscience

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Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips
 

Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips

Article DOI: 10.3791/59250-v 06:46 min May 3rd, 2019
May 3rd, 2019

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Summary

This protocol demonstrates the use of compartmentalized microfluidic chips, injection molded in a cyclic olefin copolymer to cultured neurons differentiated from human stem cells. These chips are preassembled and easier-to-use than traditional compartmentalized poly(dimethylsiloxane) devices. Multiple common experimental paradigms are described here, including viral labeling, fluidic isolation, axotomy, and immunostaining.

Tags

Compartmentalization Plastic Microfluidic Chips Neurons Stem Cells Cyclic Olefin Copolymer Chips Culture Differentiation H9 Stem Cell Line MIPSCs Secondary Containment Precoat Solution Poly-L-ornithine PBS
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