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JoVE Journal
Neuroscience

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Opdeling af humane stamcelle-afledte neuroner inden for præ-monterede plastik Microfluidic chips
 
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Opdeling af humane stamcelle-afledte neuroner inden for præ-monterede plastik Microfluidic chips

Article DOI: 10.3791/59250
May 3rd, 2019

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Summary May 3rd, 2019

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol demonstrerer brugen af opdelte mikrofluidiske chips, injektion støbt i en cyklisk olefin copolymer til dyrkede neuroner differentieret fra humane stamceller. Disse chips er færdigmonterede og lettere at bruge end traditionelle opdelte poly (dimethylsiloxan) enheder. Flere almindeligt eksperimentelle paradigmer er beskrevet her, herunder viral mærkning, Fluidic isolation, axotomi, og immun farvning.

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