Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Environment / Sample Preparation Method of Scanning and…
JoVE Journal
Environment

A subscription to JoVE is required to view this content.

JoVE Journal Environment
Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions
Journal (Environment)
A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions
Reliable Method for Assessing Seed Germination, Dormancy, and Mortality under Field Conditions
Journal (Environment)
Reliable Method for Assessing Seed Germination, Dormancy, and Mortality under Field Conditions
 

Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle

Article DOI: 10.3791/59251-v • 10:09 min • February 3rd, 2020
February 3rd, 2020 •
1,2, 3, 1, 4, 1
1The Key Laboratory for Silviculture and Conservation of the Ministry of Education, School of Forestry, Beijing Forestry University, 2School of Forestry, Inner Mongolia Agricultural University, 3Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 4School of Forestry, Fujian Agriculture and Forestry University

Chapters

Summary
Automatic Translation

In order to observe ultrastructure of insect sensilla, scanning and transmission electron microscopy (SEM and TEM, respectively) sample preparation protocol were presented in the study. Tween 20 was added into the fixative to avoid sample deformation in SEM. Fluorescence microscopy was helpful for improving slicing accuracy in TEM.

Tags

Sample Preparation Method Scanning Electron Microscope Transmission Electron Microscope Woodboring Beetle Fixative Washing Solution Body Surface Tween-20 TEM Fluorescence Microscope Slicing Accuracy Chlorophorus Caragana Field Traps Attractants Preservation Liquid Glutaraldehyde Stereo Microscope Appendages
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter