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Prøve forberedelse metode til scanning og transmission elektron mikroskop til tilhæng af træborebille
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Prøve forberedelse metode til scanning og transmission elektron mikroskop til tilhæng af træborebille

Article DOI: 10.3791/59251-v • 10:09 min • February 3rd, 2020
February 3rd, 2020 •
1,2, 3, 1, 4, 1
1The Key Laboratory for Silviculture and Conservation of the Ministry of Education, School of Forestry, Beijing Forestry University, 2School of Forestry, Inner Mongolia Agricultural University, 3Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 4School of Forestry, Fujian Agriculture and Forestry University

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For at observere ultrakonstruktion af insektsensilla blev der i undersøgelsen fremlagt en prøveforberedelsesprotokol (SEM og TEM) for at observere ultrakonstruktion af insektsensilla. Tween 20 blev tilsat i fiksativ at undgå prøvedeformation i SEM. Fluorescensmikroskopi var nyttigt til at forbedre udskæringsnøjagtigheden i TEM.

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Miljøvidenskab insekt olfaktori gustatoriske sensilla ultrastruktur scanning elektron mikroskop transmission elektron mikroskop fluorescens mikroskop kemisk økologi
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