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Journal / Neuroscience / In vivo tredimensjonale to-Foton mikroskopi å…
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JoVE Journal Neuroscience
In vivo tredimensjonale to-Foton mikroskopi å studere utført vaskulær svar av lokale ATP utstøting bruke et glass mikro-pipette
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In vivo tredimensjonale to-Foton mikroskopi å studere utført vaskulær svar av lokale ATP utstøting bruke et glass mikro-pipette

Article DOI: 10.3791/59286-v • 10:54 min • June 7th, 2019
June 7th, 2019 •
1, 1, 1, 1, 1, 1, 1,2
1Department of Neuroscience, Faculty of Medicine and Health Science, University of Copenhagen, 2Department of Clinical Neurophysiology, Rigshospitalet

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Vi presenterer en optimalisert lokal utstøting prosedyre ved hjelp av et glass mikro-pipette og en rask to-Foton hyperstack imaging metode, som tillater presis måling av kapillær diameter endringer og gransking av sin regulering i tre dimensjoner.

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Nevrovitenskap in vivo to-Foton mikroskopi cerebral blodstrøm (CBF) astrocytter tre dimensjoner (3D) nevrovaskulære kopling kalsium bildebehandling viral vektor injeksjon
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