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Biology

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JoVE Journal Biology
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Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Article DOI: 10.3791/59288-v • 12:58 min • March 26th, 2019
March 26th, 2019 •
1, 2, 2
1Core Imaging Facility and Department of Microbiology and Immunology, Loyola University Chicago, 2Department of Molecular Pharmacology and Therapeutics, Loyola University Chicago

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Summary
Automatic Translation

We describe a comprehensive and practical protocol for fluorescence recovery after photobleaching experiments with live cells. Although the protocol was used to measure the mobility of yellow fluorescent protein-tagged p62 in aggresome-like induced structures, it can be applied to a variety of microscopy systems and fluorescent proteins.

Tags

Fluorescence Recovery After Photobleaching FRAP Protocol Yellow Fluorescent Protein P62 Aggresome-like Induced Structures Protein Mobility Subcellular Compartments Live Cells Therapeutic Purposes Diagnostic Purposes Drug Delivery Systems Diffusion Phenomenon FRAP Microscopy Technique Mammalian Cells Plant Cells Yeast Cells Bacteria Image Acquisition Parameters Acquisition Region Of Interest Mobile Fraction Halftime Of Recovery
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