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Journal / Biology / Fluorescens opsving efter Photobleaching af gul…
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Fluorescens opsving efter Photobleaching af gul fluorescerende proteiner markeret p62 i Aggresome-lignende induceret strukturer
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Fluorescens opsving efter Photobleaching af gul fluorescerende proteiner markeret p62 i Aggresome-lignende induceret strukturer

Article DOI: 10.3791/59288-v • 12:58 min • March 26th, 2019
March 26th, 2019 •
1, 2, 2
1Core Imaging Facility and Department of Microbiology and Immunology, Loyola University Chicago, 2Department of Molecular Pharmacology and Therapeutics, Loyola University Chicago

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Vi beskriver en omfattende og praktisk protokol til fluorescens opsving efter photobleaching eksperimenter med levende celler. Selv om protokollen blev brugt til at måle mobilitet af gul fluorescerende proteiner-tagged p62 i aggresome-lignende menneskeskabte strukturer, kan det anvendes til en lang række mikroskopi systemer og fluorescerende proteiner.

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Biologi sag 145 fluorescens opsving efter photobleaching FRAP gul fluorescerende proteiner live celle imaging protein mobilitet murine makrofager p62 sequestosome-1 aggresome
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