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Fluorescens utvinning etter Photobleaching av gule fluorescerende Protein merket p62 i Aggresome-lignende indusert strukturer
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Fluorescens utvinning etter Photobleaching av gule fluorescerende Protein merket p62 i Aggresome-lignende indusert strukturer

Article DOI: 10.3791/59288-v • 12:58 min • March 26th, 2019
March 26th, 2019 •
1, 2, 2
1Core Imaging Facility and Department of Microbiology and Immunology, Loyola University Chicago, 2Department of Molecular Pharmacology and Therapeutics, Loyola University Chicago

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Vi beskriver en omfattende og praktisk protokoll for fluorescens gjenoppretting etter photobleaching eksperimenter med live celler. Selv om protokollen ble brukt til å måle mobilitet av gule fluorescerende protein-merket p62 i aggresome-lignende menneskeskapte strukturer, kan den brukes til en rekke mikroskopi systemer og fluorescerende proteiner.

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Biologi problemet 145 fluorescens utvinning etter photobleaching FRAP gul fluorescerende protein live celle imaging protein mobilitet murine makrofager p62 sequestosome-1 aggresome
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