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Purification de la Fraction dendritique filopodia-riche
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Purification de la Fraction dendritique filopodia-riche

Article DOI: 10.3791/59292-v • 11:51 min • May 2nd, 2019
May 2nd, 2019 •
1,2, 3
1Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, 2Liver Cancer Prevention Research Unit, RIKEN Center for Integrative Medical Sciences, 3Laboratory for Systems Molecular Ethology, RIKEN Center for Brain Science

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Dans ce protocole, nous introduisons une méthode pour purifier la fraction dendritique filopodia-riche de la structure phagocytic de la coupe-comme la saillie sur les neurones hippocampiques cultivés en profitant de l'affinité spécifique et forte entre un filopodial dendritique la molécule d'adhérence, TLCN, et une molécule extracellulaire de matrice, vitronectin.

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Neurosciences Numéro 147 filodée dendritique synapse colonne vertébrale télédencéphale ICAM-5 vitronectin neurones hippocampiques culture de faible densité microbilles magnétiques tasse phagocytique
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