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JoVE Journal Neuroscience
Rensning af den dendritiske Filopodia-rige fraktion
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Rensning af den dendritiske Filopodia-rige fraktion

Article DOI: 10.3791/59292-v • 11:51 min • May 2nd, 2019
May 2nd, 2019 •
1,2, 3
1Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, 2Liver Cancer Prevention Research Unit, RIKEN Center for Integrative Medical Sciences, 3Laboratory for Systems Molecular Ethology, RIKEN Center for Brain Science

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I denne protokol introducerer vi en metode til rensning af dendritiske filopodia-rige fraktion fra den fagocytiske Cup-lignende fremspringende struktur på dyrkede hippocampus neuroner ved at drage fordel af den specifikke og stærke affinitet mellem en dendritisk filopodial adhæsions molekyle, TLCN og et ekstracellulær matrix molekyle, vitronectin.

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Neurovidenskab dendritiske filopodia synapse rygsøjle telencepin ICAM-5 vitronectin hippocampus neuroner lav-density kultur magnetiske mikroperler Fagocytisk kop
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