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JoVE Journal
Developmental Biology

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从脐带血源性多能干细胞中提取三维皮肤有机细胞
 
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从脐带血源性多能干细胞中提取三维皮肤有机细胞

Article DOI: 10.3791/59297-v 09:54 min April 18th, 2019
April 18th, 2019

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Summary

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我们提出了一个协议, 说明如何区分诱导多能干细胞衍生角质形成细胞和成纤维细胞, 并产生三维皮肤有机体, 使用这些角质形成细胞和成纤维细胞。该协议包含生成人性化小鼠模型的附加步骤。这里介绍的技术将改进皮肤科的研究。

Tags

发育生物学 第146期 诱导多能干细胞、脐带血单个核细胞、成纤维细胞、角质形成细胞、三维皮肤有机体、植皮、人性化小鼠模型
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