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JoVE Journal
Neuroscience

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分化后再蛋白的人类多能干细胞衍生神经元,用于高含量的内尿成生和突合性成熟筛查
 
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分化后再蛋白的人类多能干细胞衍生神经元,用于高含量的内尿成生和突合性成熟筛查

Article DOI: 10.3791/59305-v 06:50 min August 28th, 2019
August 28th, 2019

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

该协议描述了一个详细的程序,用于重新悬浮和培养人类干细胞衍生神经元,这些神经元以前在体外与神经祖细胞分离了数周。该程序有助于以与光显微镜和高含量筛选相兼容的格式对神经亮物、突触和晚表达神经元标记进行基于成像的检测。

Tags

神经科学, 问题 150, iPSC, 干细胞, 再镀, 神经生成 HCS, 活力, 神经元, 神经质生长, 突触, 显微镜
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