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JoVE Journal Neuroscience
Post-differentiering Replating af humane pluripotente stamcelle-afledte neuroner for højt indhold screening af Neuritogenesis og synapse modning
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Post-differentiering Replating af humane pluripotente stamcelle-afledte neuroner for højt indhold screening af Neuritogenesis og synapse modning

Article DOI: 10.3791/59305-v • 06:50 min • August 28th, 2019
August 28th, 2019 •
1,2, 1,2, 1,2, 1,2
1Division of Biological Sciences, University of California, San Diego, 2Sanford Consortium for Regenerative Medicine

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Denne protokol beskriver en detaljeret procedure for tilbage suspendering og dyrkning af humane stamcelle afledte neuroner, der tidligere var differentieret fra neurale progenitorer in vitro i flere uger. Proceduren letter Imaging-baserede assays af neurites, synapser, og sent-udtrykker neuronal markører i et format, der er kompatibelt med lys mikroskopi og høj-indhold screening.

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Neurovidenskab iPSC stamceller replating neuritogenesis HCS levedygtighed Neuron neurite outgrowth synapser mikroskopi
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