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Journal / Biochemistry / Identificação de receptores de Orexin e…
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Biochemistry

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JoVE Journal Biochemistry
Identificação de receptores de Orexin e endocanabinoides em zebrafish adulto usando métodos de imunoperoxidase e imunofluorescência
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Identificação de receptores de Orexin e endocanabinoides em zebrafish adulto usando métodos de imunoperoxidase e imunofluorescência

Article DOI: 10.3791/59308-v • 08:37 min • June 25th, 2019
June 25th, 2019 •
1,2, 3, 3, 2, 1
1Department of Sciences and Technologies (DST), University of Sannio, 2Endocannabinoid Research Group, Institute of Biomolecular Chemistry, 3Department of Veterinary Medicine and Animal Productions, University of Naples Federico II

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São apresentados aqui protocolos para caracterização imuno-histoquímica e localização de peptídeo de Hipocretina, receptores de Hipocretina e receptores endocanabinoides no intestino e cérebros de modelos adultos de zebrafish de obesidade normal e induzida por dieta (Dio) usando immunoperixidase e métodos dobro da imunofluorescência.

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Bioquímica edição 148 Immunohistochemistry immunoperoxidase receptor do Hipocretina receptor do endocanabinóide cérebro do zebrafish intestine do zebrafish
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