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Journal / Biochemistry / Identifikation af orexin og Endocannabinoide…
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Biochemistry

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JoVE Journal Biochemistry
Identifikation af orexin og Endocannabinoide receptorer hos voksne zebrafisk ved hjælp af Immunoperoxidase og immunofluorescens metoder
Purification of Ubiquitinated p53 Proteins from Mammalian Cells
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Purification of Ubiquitinated p53 Proteins from Mammalian Cells
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Identifikation af orexin og Endocannabinoide receptorer hos voksne zebrafisk ved hjælp af Immunoperoxidase og immunofluorescens metoder

Article DOI: 10.3791/59308-v • 08:37 min • June 25th, 2019
June 25th, 2019 •
1,2, 3, 3, 2, 1
1Department of Sciences and Technologies (DST), University of Sannio, 2Endocannabinoid Research Group, Institute of Biomolecular Chemistry, 3Department of Veterinary Medicine and Animal Productions, University of Naples Federico II

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Præsenteret her er protokoller for immunhistokemiske karakterisering og lokalisering af orexin peptid, orexin receptorer, og endocannabinoide receptorer i tarmen og hjernen af normale og kost-induceret fedme (Dio) voksne: zebrafisk modeller ved hjælp af immunoperixidase og dobbelte immunofluorescens metoder.

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Biokemi Immunohistokemi immunoperoxidase orexin receptor endocannabinoide receptor zebrafiskhjerne zebrafisktarm
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