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Journal / Developmental Biology / Real-Time Live-cel Stroom Cytometry te…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Real-Time Live-cel Stroom Cytometry te onderzoeken Calcium toestroom, porie vorming en fagocytose door P2X7 receptoren in volwassen neurale voorlopercellen
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Real-Time Live-cel Stroom Cytometry te onderzoeken Calcium toestroom, porie vorming en fagocytose door P2X7 receptoren in volwassen neurale voorlopercellen

Article DOI: 10.3791/59313-v • 11:47 min • April 3rd, 2019
April 3rd, 2019 •
1,2, 3,4, 3,5,6, 7, *1,4,7, *8
1Griffith Institute for Drug Discovery, Griffith University, 2Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 3Discipline of Anatomy and Histology, School of Medical Science, University of Sydney, 4Bosch Institute, University of Sydney, 5Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit, St. Vincent's Centre for Applied Medical Research, 6School of Medical Sciences, The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, 7School of Environment and Science, Griffith University, Brisbane, Queensland, 8Florey Institute of Neuroscience and Mental Health, University of Melbourne
* These authors contributed equally

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Verstrekken van eencellige gevoeligheid, is real-time stroom cytometry uniek geschikt voor het kwantificeren van multimodale receptor functies van levende culturen. Met behulp van neurale volwassen voorlopercellen, de P2X7 receptor functie evalueerden via calcium toestroom gedetecteerd door calcium indicator kleurstof, transmembraan porie vorming door ethidium bromide opname en fagocytose met behulp van fluorescerende latex kralen.

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Ontwikkelingsbiologie kwestie 146 P2X7 receptoren stroom cytometry calcium purinergic signalering fagocytose porie vorming neurale voorlopercellen
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