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Immunology and Infection

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JoVE Journal Immunology and Infection
Billedbehandling protokol for analysen af Diffusion og Cluster størrelse af membranreceptorer af Fluorescens mikroskopi
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Billedbehandling protokol for analysen af Diffusion og Cluster størrelse af membranreceptorer af Fluorescens mikroskopi

Article DOI: 10.3791/59314-v • 12:15 min • April 9th, 2019
April 9th, 2019 •
*1,2, *3,4, 3,5, 3, 1,6, 3, 3
1Department of Macromolecular Structures, Centro Nacional de Biotecnología, Campus Univ. Autónoma de Madrid, 2Campus Urb. Montepríncipe s/n, Univ. San Pablo CEU, 3Department of Immunology and Oncology, Centro Nacional de Biotecnología, Campus Univ. Autónoma de Madrid, 4Department of Cell Signaling, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CSIC), 5Meyer Cancer Center, 6Department of Anatomy and Cell Biology, McGill Univ.
* These authors contributed equally

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Vi præsenterer her, en protokol for enkelt partikel tracking billedanalyse, der giver mulighed for kvantitative evaluering af diffusion koefficienter, typer af motion og klynge størrelser af én partikler opdaget af Fluorescens mikroskopi.

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Immunologi og infektion spørgsmålet 146 SPT chemokine receptor sporing klynger diffusion analyse software
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