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Conversão de pilhas de haste pluripotentes induzidas humanas (iPSCs) em neurônios de motor espinal e craniano funcionais usando vetores de PiggyBac
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Conversão de pilhas de haste pluripotentes induzidas humanas (iPSCs) em neurônios de motor espinal e craniano funcionais usando vetores de PiggyBac

Article DOI: 10.3791/59321-v • 07:33 min • May 1st, 2019
May 1st, 2019 •
1, 2, 2, 2, 3, 2,4, 2,4, 1,2
1Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Italy, 2Center for Life Nano Science, Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology, The Rockefeller University, USA, 4Department of Physiology and Pharmacology, Sapienza University of Rome, Italy

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Este protocolo permite a conversão rápida e eficiente de pilhas de haste pluripotentes induzidas em neurônios de motor com uma identidade espinal ou craniana, pela expressão ectópica de fatores da transcrição dos vetores induzível de piggybac.

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Neurociência células-tronco pluripotentes induzidas neurônios motores piggyBac diferenciação fatores de transcrição Phox2a Isl1 Ngn2 Lhx3
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