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Journal / Biochemistry / A Strategy to Identify Compounds that Affect Cell …
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Biochemistry

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JoVE Journal Biochemistry
A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput
Cell Seeding and Transfection on Electron Microscopy (EM) Grids for Cryotomography
Journal (Biochemistry)
Cell Seeding and Transfection on Electron Microscopy (EM) Grids for Cryotomography
Oil and Crystallization Cocktail Well Plate Preparation
Journal (Biochemistry)
Oil and Crystallization Cocktail Well Plate Preparation
 

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

Article DOI: 10.3791/59333-v • 12:22 min • September 22nd, 2019
September 22nd, 2019 •
1, 1, 1, 1
1National Institute of Neurological Disorders and Stroke (NINDS), Neurotherapeutic Development Unit (NTDU), National Institutes of Health (NIH)

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Summary
Automatic Translation

It is often necessary to assess the potential cytotoxicity of a set of compounds on cultured cells. Here, we describe a strategy to reliably screen for toxic compounds in a 96-well format.

Tags

Cell Growth Cell Survival Compound Screening Cytotoxic Compounds Assay Cellular Function Dose Response Curve Primary Screen Cell Culture T75 Flask NSC Medium Incubator
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