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Journal / Biochemistry / En strategi til identificering af forbindelser,…
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Biochemistry

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JoVE Journal Biochemistry
En strategi til identificering af forbindelser, der påvirker cellevækst og overlevelse i dyrkede pattedyrceller ved lav til moderat gennemløb
Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Journal (Biochemistry)
Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis
Journal (Biochemistry)
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En strategi til identificering af forbindelser, der påvirker cellevækst og overlevelse i dyrkede pattedyrceller ved lav til moderat gennemløb

Article DOI: 10.3791/59333-v • 12:22 min • September 22nd, 2019
September 22nd, 2019 •
1, 1, 1, 1
1National Institute of Neurological Disorders and Stroke (NINDS), Neurotherapeutic Development Unit (NTDU), National Institutes of Health (NIH)

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Det er ofte nødvendigt at vurdere den potentielle cytotoksicitet af et sæt forbindelser på dyrkede celler. Her beskriver vi en strategi for pålideligt at screene for giftige forbindelser i et 96-Well-format.

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Biokemi toksicitets analyse cytotoksicitet 3-(4,5-dimethylthizol-2-yl)-2 5diphenyltetrazoliumbromid MTT moderat gennemløb dyrkede pattedyrsceller fluorescerende billeddannelse celletælling
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