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Journal / Bioengineering / CRISPR/Cas12a multiplex genom redigering av…
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Bioengineering

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JoVE Journal Bioengineering
CRISPR/Cas12a multiplex genom redigering av Saccharomyces cerevisiae och skapandet av jäst pixel art
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CRISPR/Cas12a multiplex genom redigering av Saccharomyces cerevisiae och skapandet av jäst pixel art

Article DOI: 10.3791/59350-v • 10:18 min • May 28th, 2019
May 28th, 2019 •
1,2, 1, 3,4, 1, 1
1DSM Biotechnology Center, 2Biochemistry and Molecular Biology, Department of Chemistry, University of Hamburg, 3BrisSynBio, University of Bristol, Life Sciences Building, 4School of Biological Sciences, University of Bristol, Life Sciences Building

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CRISPR/Cas12a-systemet i kombination med en enda crRNA-array möjliggör effektiv multiplex-redigering av S. cerevisiae -genomet vid flera loci samtidigt. Detta demonstreras genom att konstruera karotenoid producerar jäststammar som sedan används för att skapa jäst pixel konst.

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Bioteknik CRISPR/Cas12a CRISPR/Cpf1 CRISPR/Cas9 multiplex genom redigering Saccharomyces cerevisiae jäst pixel art
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