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Kromatin immunoprecipitation analys med hjälp av Micrococcal nukleaser i däggdjursceller

Article DOI: 10.3791/59375-v • 11:42 min • May 10th, 2019
May 10th, 2019 •
1, 1
1Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope

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Kromatin immunoprecipitation (ChIP) är ett kraftfullt verktyg för att förstå de molekylära mekanismerna för genreglering. Metoden innebär dock svårigheter att få reproducerbar kromatinfragmentering genom mekanisk klippning. Här ger vi ett förbättrat protokoll för en ChIP-analys med enzymatisk matsmältning.

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Genetik genreglering histonmodifiering kromatin immunoprecipitation micrococcal Nuclease RNA-polymeras II trimetylerad Histon H3 lysin 4
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