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Journal / Genetics / 使用哺乳动物细胞中的微球菌核酸酶进行染色质免疫沉淀测定…
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JoVE Journal Genetics
使用哺乳动物细胞中的微球菌核酸酶进行染色质免疫沉淀测定
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使用哺乳动物细胞中的微球菌核酸酶进行染色质免疫沉淀测定

Article DOI: 10.3791/59375-v • 11:42 min • May 10th, 2019
May 10th, 2019 •
1, 1
1Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope

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染色质免疫沉淀(ChIP)是了解基因调控的分子机制的有力工具。然而,该方法在通过机械剪切获得可重复染色质碎片方面遇到了困难。在这里,我们为使用酶消化的ChIP测定提供了改进的协议。

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遗传学 问题 147 基因调控 组蛋白修饰 染色质免疫沉淀 微球核酸 RNA聚合酶 II 三甲基化组蛋白 H3 莱辛 4
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