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Chapters
- 00:04Title
- 00:49The High Titer sgRNA Library Lentivirus Generation
- 01:44Titration of Lentiviral Library in MOLM13 Leukemia Cells
- 02:59Transduction of the Pooled CRISPR-Cas9 KO Library
- 04:20Screening of the Pooled CRISPR-Cas9 KO Library with One-Step RT-qPCR
- 05:41Verification of Integrated sgRNAs Positive Clones through Genotyping and Sanger Sequence
- 06:37Detection of sgRNAs Induced Indel Mutation by Nuclease Digestion Assay
- 07:48Results: Pooled CRISPR-Cas9 KO Library Screening and Identification
- 09:19Conclusion
En CRISPR/sgRNA bibliotek er utlignet til avhør protein-koding gener. Muligheten for et sgRNA-bibliotek for å avdekke funksjonen til en CTCF grense i genet regulering imidlertid uutforsket. Her beskriver vi et HOX loci bestemte sgRNA bibliotek for å belyse funksjon CTCF grenser i HOX loci.
