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Cancer Research

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Predicting In Vivo Payloads Delivery using a Blood-brain Tumor-barrier in a Dish
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Predicting In Vivo Payloads Delivery using a Blood-brain Tumor-barrier in a Dish

Article DOI: 10.3791/59384-v • 13:34 min • April 16th, 2019
April 16th, 2019 •
1, 1,2, 1,3
1Research Programs Unit, Translational Cancer Medicine Research Program, University of Helsinki, 2Wihuri Research Institute, Biomedicum Helsinki, University of Helsinki, 3Laboratory Animal Center, Helsinki Institute of Life Science (HiLIFE), University of Helsinki

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Summary
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Drug targeting to central nervous system tumors is a major challenge. Here we describe a protocol to produce an in vitro mimic of the blood-brain tumor-barrier using murine and/or human cells and discuss their relevance for the predictability of central nervous system tumor targeting in vivo.

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Involves Careful Handling And Positioning. The Use Of Fluorescent Dyes And Microscopy Techniques Allows For Real-time Visualization And Assessment Of The Compounds' Diffusion And Targeting Capabilities. This Method Provides Valuable Insights Into The Effectiveness Of Drug Delivery To The Brain Tumor Site Helping Researchers Make Informed Decisions In Drug Development. Blood-brain Barrier In Vitro Model Drug Delivery Tumor Targeting Cell Lines Reproducibility Preclinical Models Patient-derived Cells Drug Diffusion Drug Vehicles Fluorescent Dyes Microscopy Techniques
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