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In vitro Ubiquitination en Deubiquitinisatie assays van Nucleosomale histonen
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In vitro Ubiquitination en Deubiquitinisatie assays van Nucleosomale histonen

Article DOI: 10.3791/59385-v • 11:36 min • July 25th, 2019
July 25th, 2019 •
*1, *1, 1, 1, 1, 1, 1, 1,2, 1,2
1Maisonneuve-Rosemont Hospital Research Center and Department of Medicine, University of Montréal, 2Lunenfeld-Tanenbaum Research Institute, Sinai Health System
* These authors contributed equally

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Ubiquitination is een post-translationele modificatie die belangrijke rollen speelt in cellulaire processen en nauw gecoördineerd wordt door deubiquitination. Defecten in beide reacties ten grondslag liggen aan menselijke pathologieën. Wij bieden protocollen voor het uitvoeren van Ubiquitination en deubiquitinisatie reactie in vitro met behulp van gezuiverde componenten.

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Biochemie uitgave 149 alomtegenwoordige deubiquitination E3 ubiquitine ligase PRC1 deubiquitinase PR-DUB chromatin BAP1 ASXL BMI1 RING1B H2A
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