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JoVE Journal
Biochemistry

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In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones In Vitro
 
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In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones In Vitro

Article DOI: 10.3791/59385-v 11:36 min July 25th, 2019
July 25th, 2019

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Summary

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L'ubiquitination est une modification post-traductionnelle qui joue un rôle important dans les processus cellulaires et est étroitement coordonnée par la désubiquitination. Les défauts dans les deux réactions sous-tendent les pathologies humaines. Nous fournissons des protocoles pour mener la réaction d'ubiquitination et de deubiquitination in vitro utilisant des composants purifiés.

Tags

Biochimie Numéro 149 ubiquitination deubiquitination E3 ubiquitin ligase PRC1 deubiquitinase PR-DUB chromatin BAP1 ASXL BMI1 RING1B H2A
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