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Biochemistry

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JoVE Journal Biochemistry
In Vitro Ubiquitination e Deubiquitination Saggi di Istoni Nucleosomici
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In Vitro Ubiquitination e Deubiquitination Saggi di Istoni Nucleosomici

Article DOI: 10.3791/59385-v • 11:36 min • July 25th, 2019
July 25th, 2019 •
*1, *1, 1, 1, 1, 1, 1, 1,2, 1,2
1Maisonneuve-Rosemont Hospital Research Center and Department of Medicine, University of Montréal, 2Lunenfeld-Tanenbaum Research Institute, Sinai Health System
* These authors contributed equally

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L'ubiquitinazione è una modifica post-traduzionale che svolge un ruolo importante nei processi cellulari ed è strettamente coordinata dalla deubiquitinazione. I difetti in entrambe le reazioni sono alla base delle patologie umane. Forniamo protocolli per condurre l'ubiquitinazione e la reazione di deubiquitinazione in vitro utilizzando componenti purificati.

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Biochimica Numero 149 ubiquitinazione deubiquitinazione E3 ubiquitin ligase PRC1 deubiquitinase PR-DUB cromatina BAP1 ASXL BMI1 RING1B H2A
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