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Journal / Biochemistry / In vitro Ubiquitination och Deubiquitination…
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JoVE Journal Biochemistry
In vitro Ubiquitination och Deubiquitination assays av Nukleosomala Histones
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Journal (Biochemistry)
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In vitro Ubiquitination och Deubiquitination assays av Nukleosomala Histones

Article DOI: 10.3791/59385-v • 11:36 min • July 25th, 2019
July 25th, 2019 •
*1, *1, 1, 1, 1, 1, 1, 1,2, 1,2
1Maisonneuve-Rosemont Hospital Research Center and Department of Medicine, University of Montréal, 2Lunenfeld-Tanenbaum Research Institute, Sinai Health System
* These authors contributed equally

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Ubiquitination är en post-translationella modifiering som spelar viktiga roller i cellulära processer och är tätt koordinerad av deubiquitination. Defekter i båda reaktionerna ligger bakom mänskliga patologier. Vi tillhandahåller protokoll för att utföra ubiquitination och deubiquitination reaktion in vitro med hjälp av renade komponenter.

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Biokemi fråga 149 ubiquitination deubiquitination E3 ubiquitin Ligase PRC1 deubiquitinase PR-DUB Chromatin BAP1 ASXL BMI1 RING1B H2A
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