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Journal / Bioengineering / Visualisierung von Germinosomes und die innere…
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JoVE Journal Bioengineering
Visualisierung von Germinosomes und die innere Membran in Bacillus Subtilis Sporen
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Visualisierung von Germinosomes und die innere Membran in Bacillus Subtilis Sporen

Article DOI: 10.3791/59388-v • 08:58 min • April 15th, 2019
April 15th, 2019 •
1, 1, *2,3, *4, *1
1Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, 2Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, 3Confocal.nl BV., 4Dept. of Molecular Biology and Biophysics, UConn Health
* These authors contributed equally

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Germinant Rezeptor-Proteine-Cluster in "Germinosomes" in der inneren Membran der Bacillus Subtilis Sporen. Wir beschreiben ein Protokoll mit Superresolution Mikroskopie und Leuchtstoffröhren Reporter Proteine, um Germinosomes zu visualisieren. Das Protokoll zeigt auch Spore innere Membrandomänen, die bevorzugt mit dem Membran-Farbstoff FM4-64 gefärbt sind.

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Bioengineering Ausgabe 146 Gram-positiven Bakterien Bacillales Mikroskopie Mikrobiologie Organismen Bakterien analytische diagnostische und therapeutische Techniken und Geräte Untersuchungstechniken Biowissenschaften Life Sciences (General) Bacillus Sporen keimen Proteine Germinosomes Spore innere Membran super Auflösung Mikroskopie Fluoreszenz-Mikroskopie
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