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Journal / Bioengineering / Visualisering av Germinosomes och det inre…
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Bioengineering

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JoVE Journal Bioengineering
Visualisering av Germinosomes och det inre membranet i Bacillus subtilis sporer
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Visualisering av Germinosomes och det inre membranet i Bacillus subtilis sporer

Article DOI: 10.3791/59388-v • 08:58 min • April 15th, 2019
April 15th, 2019 •
1, 1, *2,3, *4, *1
1Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, 2Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, 3Confocal.nl BV., 4Dept. of Molecular Biology and Biophysics, UConn Health
* These authors contributed equally

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Germinant receptor protein kluster i 'germinosomes' i det inre membranet av Bacillus subtilis sporer. Vi beskriver ett protokoll med super upplösning Mikroskopi och fluorescerande reporter proteiner visualisera germinosomes. Protokollet identifierar också spore inre membranet domäner som är prioriterat fläckade membran färgämnet FM4-64.

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Bioteknik fråga 146 grampositiva bakterier Bakteriologistubbar mikroskopi mikrobiologi organismer bakterier analytisk diagnostiska och terapeutiska tekniker och utrustning utredningsteknik Life sciences Life Sciences (allmänt) Bacillus Sporer grobarhet proteiner Germinosomes Spore inre membran super resolution mikroskopi fluorescensmikroskopi
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