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Journal / Bioengineering / Visualizzazione di Germinosomes e la membrana…
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Bioengineering

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JoVE Journal Bioengineering
Visualizzazione di Germinosomes e la membrana interna in Bacillus subtilis spore
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Visualizzazione di Germinosomes e la membrana interna in Bacillus subtilis spore

Article DOI: 10.3791/59388-v • 08:58 min • April 15th, 2019
April 15th, 2019 •
1, 1, *2,3, *4, *1
1Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, 2Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, 3Confocal.nl BV., 4Dept. of Molecular Biology and Biophysics, UConn Health
* These authors contributed equally

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Cluster di proteine recettoriali germinant in 'germinosomes' nella membrana interna di spore di Bacillus subtilis . Descriviamo un protocollo utilizzando super risoluzione di microscopia e fluorescenti di proteine reporter per visualizzare germinosomes. Il protocollo identifica anche domini di membrana interna di spore che sono preferenzialmente colorate con il colorante di membrana FM4-64.

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Bioingegneria problema 146 batteri Gram-positivi Bacillales microscopia microbiologia organismi batteri analitica diagnostico e terapeutico tecniche e attrezzature tecniche Investigative scienze biologiche Scienze della vita (generale) Bacillus Spore proteine di germinazione Germinosomes membrana interna di Spore super microscopia ad alta risoluzione microscopia a fluorescenza
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