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Biochemistry

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JoVE Journal Biochemistry
Direkte måling af KDM1A Target engagement ved hjælp af Chemoprobe-baserede Immunoassays
Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
Journal (Biochemistry)
Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
Journal (Biochemistry)
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Direkte måling af KDM1A Target engagement ved hjælp af Chemoprobe-baserede Immunoassays

Article DOI: 10.3791/59390-v • 11:17 min • June 13th, 2019
June 13th, 2019 •
1, 1, 1
1Oryzon Genomics

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Her præsenterer vi en protokol til måling KDM1A Target engagement i en menneske-eller dyrecelle, væv eller blodprøver behandlet med KDM1A-hæmmere. Protokollen anvender chemoprobe-mærkning af det frie KDM1A enzym og direkte kvantificering af målbesættelsen ved hjælp af chemoprobe-baserede immunassays og kan anvendes i prækliniske og kliniske studier.

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Biokemi Target engagement Histone modifikationer Histone Demethylases KDM1A Chemoprobe ELISA
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