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Journal / Biochemistry / Nitropeptid profilering og identifikation…
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Biochemistry

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JoVE Journal Biochemistry
Nitropeptid profilering og identifikation illustreret af angiotensin II
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Nitropeptid profilering og identifikation illustreret af angiotensin II

Article DOI: 10.3791/59391-v • 07:31 min • June 16th, 2019
June 16th, 2019 •
1,2, 3, 2,4,5,6
1Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 2Department of Biological Sciences, University of Notre Dame, 3Department of Urology, Shanghai East Hospital, Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, 5Harper Cancer Research Institute, University of Notre Dame, 6Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Cancer Center

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Proteomisk profilering af tyrosin-nitrerede proteiner har været en udfordrende teknik på grund af den lave overflod af 3-nitrotyrosin modifikation. Her beskriver vi en ny metode til tilsætning af nitropeptid og profilering ved hjælp af angiotensin II som model. Denne metode kan udvides til andre in vitro -eller in vivo -systemer.

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Biokemi Nitropeptid angiotensin II nitrotyrosin dimethylmærkning natriumdithionat biotin mærkning berigelse
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