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JoVE Journal Biochemistry
Perfil e identificação de nitropeptídeos ilustrados pela angiotensina II
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Perfil e identificação de nitropeptídeos ilustrados pela angiotensina II

Article DOI: 10.3791/59391-v • 07:31 min • June 16th, 2019
June 16th, 2019 •
1,2, 3, 2,4,5,6
1Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 2Department of Biological Sciences, University of Notre Dame, 3Department of Urology, Shanghai East Hospital, Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, 5Harper Cancer Research Institute, University of Notre Dame, 6Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Cancer Center

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O perfilamento Proteomic de proteínas tyrosine-nitrated foi uma técnica desafiante devido à baixa abundância da modificação da 3-nitrotyrosine. Aqui nós descrevemos uma aproximação nova para o enriquecimento e o perfilamento do nitropeptide usando a angiotensina II como o modelo. Este método pode ser estendido para outros sistemas in vitro ou in vivo .

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Bioquímica edição 148 Nitropeptídeo angiotensina II nitrotirosina rotulagem de dimetil dithionate de sódio rotulagem de biotina enriquecimento
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