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Journal / Biochemistry / Anjiyotensin II tarafından gösterilen nitropeptid …
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Biochemistry

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JoVE Journal Biochemistry
Anjiyotensin II tarafından gösterilen nitropeptid profil oluşturma ve tanımlama
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Anjiyotensin II tarafından gösterilen nitropeptid profil oluşturma ve tanımlama

Article DOI: 10.3791/59391-v • 07:31 min • June 16th, 2019
June 16th, 2019 •
1,2, 3, 2,4,5,6
1Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 2Department of Biological Sciences, University of Notre Dame, 3Department of Urology, Shanghai East Hospital, Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, 5Harper Cancer Research Institute, University of Notre Dame, 6Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Cancer Center

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Tirozin-nitrated proteinlerin proteomik profilleme 3-nitrotirozin modifikasyonunda düşük bolluk nedeniyle zorlu bir teknik olmuştur. Burada, nitropeptid zenginleştirme ve model olarak angiotensin II kullanarak profil oluşturma için yeni bir yaklaşım açıklanmaktadır. Bu yöntem diğer in vitro veya in vivo sistemlerde uzatılabilir.

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Biyokimya Sayı 148 Nitropeptid Anjiyotensin II nitrotirozin dimetil etiketleme sodyum dithionate biotin etiketleme zenginleştirme
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