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Journal / Immunology and Infection / Afhører individuelle Autoreactive Kimcentrene af…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Afhører individuelle Autoreactive Kimcentrene af Photoactivation i en blandet kimære Model af autoimmunitet
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Afhører individuelle Autoreactive Kimcentrene af Photoactivation i en blandet kimære Model af autoimmunitet

Article DOI: 10.3791/59397-v • 11:12 min • April 11th, 2019
April 11th, 2019 •
1, 1, 1
1Department of Biomedicine, Aarhus University

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Click here for the English version.

Denne protokol beskriver generation af blandet murine knoglemarv kimærer med spontane autoimmune Kimcentre, i hvilke autoreactive lymfocytter bære en photoactivatable grøn fluorescerende proteiner (PA-NGL) reporter. Dette giver mulighed for at samkøre cellular placering i væv med downstream molekylære og funktionelle analyser.

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Immunologi og infektion spørgsmålet 146 Photoactivation photoactivatable grøn fluorescerende proteiner (PA-NGL) autoimmunitet milt lymfeknude mus eksplantat blandet knoglemarv chimera to-foton mikroskopi
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