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Journal / Neuroscience / 3D-billeddannelse i høj opløsning af rabies virus …
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Neuroscience

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JoVE Journal Neuroscience
3D-billeddannelse i høj opløsning af rabies virus infektion i et solvent renset hjernevæv
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3D-billeddannelse i høj opløsning af rabies virus infektion i et solvent renset hjernevæv

Article DOI: 10.3791/59402-v • 09:42 min • April 30th, 2019
April 30th, 2019 •
1, 1, 1, 1, 1
1Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health

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Roman, immun farvning-kompatible vævs clearing teknikker som den ultimative 3D-billeddannelse af solvent-clearede organer tillader 3D visualisering af rabiesvirus hjerneinfektion og dens komplekse cellulære miljø. Tykke, antistof-mærkede hjernevæv skiver er lavet optisk gennemsigtige for at øge billeddannelse dybde og for at muliggøre 3D-analyse af konfokale Laserscanning mikroskopi.

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Neurovidenskab Confokal Laserscanning mikroskopi uDISCO iDISCO immunofluorescens vævs clearing optisk clearing Deep-vævs antistof mærkning 3D imaging rabiesvirus hjernen Neuro invasion patogenesen
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