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JoVE Journal Neuroscience
Hoge-resolutie 3D-beeldvorming van rabiës virus infectie in solvent-geklaard hersenweefsel
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Hoge-resolutie 3D-beeldvorming van rabiës virus infectie in solvent-geklaard hersenweefsel

Article DOI: 10.3791/59402-v • 09:42 min • April 30th, 2019
April 30th, 2019 •
1, 1, 1, 1, 1
1Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health

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Roman, immunokleuring-compatibele weefsel clearing technieken zoals de ultieme 3D-beeldvorming van solvent-geclearde organen toestaan de 3D-visualisatie van rabiësvirus herseninfectie en zijn complexe cellulaire omgeving. Dikke, antilichaam gelabelde hersenweefsel segmenten zijn optisch transparant gemaakt om de beeld diepte te vergroten en 3D-analyse mogelijk te maken door confocale laser scanning microscopie.

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Neuroscience probleem 146 confocale laser scanning microscopie uDISCO iDISCO immunofluorescentie weefsel clearing optische clearing Deep-tissue antilichamen labeling 3D-beeldvorming rabiësvirus hersenen neuro invasie pathogenese
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