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Journal / Genetics / 单分子荧光原位杂交 (SM-FISH) 在小鼠卵母细胞中 Mrna…
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JoVE Journal Genetics
单分子荧光原位杂交 (SM-FISH) 在小鼠卵母细胞中 Mrna 的定量和定位
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单分子荧光原位杂交 (SM-FISH) 在小鼠卵母细胞中 Mrna 的定量和定位

Article DOI: 10.3791/59414-v • 08:18 min • April 24th, 2019
April 24th, 2019 •
1, 1
1Department of Animal Science, University of Nebraska-Lincoln

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为了可重复地计数单个卵母细胞中的 Mrna 数量, 对非粘附细胞的单分子 RNA 荧光原位杂交 (RNA-FISH) 进行了优化。卵母细胞被收集, 与转录特异性探针杂交, 并使用图像定量软件进行量化。

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遗传学 第146期 卵母细胞 mRNA 定量 mRNA 定位 非粘附细胞 荧光原位杂交 分支 dna
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