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Journal / Genetics / Bruk av ett molekyl fluorescerende i Situ…
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JoVE Journal Genetics
Bruk av ett molekyl fluorescerende i Situ hybridisering (SM-fisk) å kvantifisere og Localize mRNAs i Murine Oocytes
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Bruk av ett molekyl fluorescerende i Situ hybridisering (SM-fisk) å kvantifisere og Localize mRNAs i Murine Oocytes

Article DOI: 10.3791/59414-v • 08:18 min • April 24th, 2019
April 24th, 2019 •
1, 1
1Department of Animal Science, University of Nebraska-Lincoln

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Du reproduserbar teller antallet mRNAs i individuelle oocytes, ett molekyl RNA fluorescens i situ var hybridisering (RNA-fisk) optimalisert for ikke-tilhenger celler. Oocytes var samlet, hybridiserte med transkripsjon bestemt sonder og kvantifisert bruker en kvantifisering programvare.

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Genetikk problemet 146 Oocyte mRNA kvantifisering mRNA lokalisering ikke-tilhenger celler fluorescens i situ hybridisering forgrenede DNA
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