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JoVE Journal
Immunology and Infection

This content is Open Access.

Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir Assessment
 

Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir Assessment

Article DOI: 10.3791/59427-v 07:21 min June 23rd, 2019
June 23rd, 2019

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Summary

We describe a method for processing bronchoalveolar lavage fluid and matched peripheral blood from chronically HIV-infected individuals on antiretroviral therapy to assess pulmonary HIV reservoirs. These methods result in the acquisition of highly pure CD4 T cells and alveolar macrophages that may subsequently be used for immunophenotyping and HIV DNA/RNA quantifications by ultrasensitive polymerase chain reaction.

Tags

HIV Reservoir Assessment Alveolar Macrophages CD4+ T-cells Immune Cells Bronchoalveolar Lavage Fluid HIV Persistence Pulmonary Mucosal T Cells Virologic Assessment Cellular Reservoirs Primary Macrophages Inflammatory Pulmonary Diseases Infectious Pulmonary Diseases Asthma Tuberculosis BAL Fluid Processing
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