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Tecnologia CRISPR/Cas9 na restauração da expressão de distrofina em progenitores musculares derivados de iPSC
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Tecnologia CRISPR/Cas9 na restauração da expressão de distrofina em progenitores musculares derivados de iPSC

Article DOI: 10.3791/59432-v • 07:44 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1, 1, 1
1Medical College of Georgia, Augusta University

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Aqui, nós apresentamos um protocolo de apagamento exon23 baseado em Cas9 para restaurar a expressão de distrofina em iPSC de fibroblastos de pele derivados do mouseMDX DMD e diferenciar diretamente iPSCs em células progenitoras miogênicas (MPC) usando o sistema de ativação Tet-on MyoD.

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Biologia do desenvolvimento células-tronco pluripotentes induzidas iPSC CRISPR/Cas9 DMD distrofina progenitor miogênico diferenciação
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