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Developmental Biology

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JoVE Journal Developmental Biology
Tecnologia CRISPR/Cas9 nel ripristino dell'espressione della distrofina nei progenitori muscolari derivati da iPSC
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Tecnologia CRISPR/Cas9 nel ripristino dell'espressione della distrofina nei progenitori muscolari derivati da iPSC

Article DOI: 10.3791/59432-v • 07:44 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1, 1, 1
1Medical College of Georgia, Augusta University

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Qui, presentiamo un protocollo di eliminazione exon23 basato su Cas9 per ripristinare l'espressione distrofina in iPSC da fibroblasti cutanei derivati da mousemdx Dmd e differenziare direttamente gli iPSC in cellule progenitrici miogenie (MPC) utilizzando il sistema di attivazione Tet-on MyoD.

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Biologia dello sviluppo numero 151 cellule staminali pluripotenti indotte iPSC CRISPR/Cas9 DMD distrofina progenitore miogenico differenziazione
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