Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Tecnología CRISPR/Cas9 en la restauración de la…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
Tecnología CRISPR/Cas9 en la restauración de la expresión de distrofina en progenitores musculares derivados de iPSC
Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants
Journal (Developmental Biology)
Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants
A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells
Journal (Developmental Biology)
A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells
 
Click here for the English version

Tecnología CRISPR/Cas9 en la restauración de la expresión de distrofina en progenitores musculares derivados de iPSC

Article DOI: 10.3791/59432-v • 07:44 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1, 1, 1
1Medical College of Georgia, Augusta University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Aquí, presentamos un protocolo de eliminación exon23 basado en Cas9 para restaurar la expresión de distrofina en iPSC a partir de fibroblastos cutáneos derivados del ratón Dmdmdx y diferenciar directamente los iPSC en células progenitoras miogénicas (MPC) utilizando el sistema de activación Tet-on MyoD.

Tags

Biología del desarrollo número 151 células madre pluripotentes inducidas iPSC CRISPR/Cas9 DMD distrofina progenitor miogénico diferenciación
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter