Modified Heterotopic Hindlimb Osteomyocutaneous Flap Model in the Rat for Translational Vascularized Composite Allotransplantation Research

Yoram Fleissig; Robert  M. Reed; Jason E. Beare; Amanda  J. LeBlanc; Stuart  K. Williams; Christina  L. Kaufman; James  B. Hoying

doi:10.3791/59458

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Modified Heterotopic Hindlimb Osteomyocutaneous Flap Model in the Rat for Translational Vascularized Composite Allotransplantation Research

JoVE Journal
Medicine

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JoVE Journal Medicine

Modified Heterotopic Hindlimb Osteomyocutaneous Flap Model in the Rat for Translational Vascularized Composite Allotransplantation Research

5,737 Views

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09:36 min

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April 26, 2019

DOI:

10.3791/59458-v

DOI:

10.3791/59458-v

•

09:36 min

April 26, 2019

•

3 Views

Yoram Fleissig¹, Robert M. Reed^2,3, Jason E. Beare^2,3, Amanda J. LeBlanc^2,4, Stuart K. Williams^2,4, Christina L. Kaufman¹, James B. Hoying^2,4

¹Christine M. Kleinert Institute for Hand and Microsurgery, ²Cardiovascular Innovation Institute,University of Louisville, ³Kentucky Spinal Cord Injury Research Center,University of Louisville, ⁴Department of Physiology,University of Louisville

Transcript

Summary

Vascularized composite allograft offers life-altering benefits to transplant recipients, but the biological causes of graft rejection and vasculopathy remain poorly understood. The rodent surgical model presented here offers a reproducible, clinically relevant model of transplantation, allowing researchers to evaluate rejection events and potential therapeutic strategies to prevent their occurrence.