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June 17, 2019
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Summary
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Questo protocollo mira a generare interneuroni direttamente riprogrammati in vivo, utilizzando un sistema virale basato su AAV nel cervello e un giornalista GFP basato sulla sinapsi FLEX, che consente l'identificazione delle cellule e ulteriori analisi in vivo.
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Cite this Article
Pereira, M., Birtele, M., Rylander Ottosson, D. In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors. J. Vis. Exp. (148), e59465, doi:10.3791/59465 (2019).
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