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Journal / Neuroscience / In vivo direkt omprogrammering av bosatta…
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Neuroscience

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JoVE Journal Neuroscience
In vivo direkt omprogrammering av bosatta gliaceller i interneuroner genom intracerebral injektion av virala vektorer
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In vivo direkt omprogrammering av bosatta gliaceller i interneuroner genom intracerebral injektion av virala vektorer

Article DOI: 10.3791/59465-v • 12:26 min • June 17th, 2019
June 17th, 2019 •
1, 1, 1
1Department of Experimental Medical Sciences and Lund Stem Cell Center, BMC, Lund University

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Detta protokoll syftar till att generera direkt omprogrammeras interneuroner in vivo, med hjälp av en AAV-baserade virus system i hjärnan och en FLEX synapsin-driven GFP reporter, som möjliggör cell identifiering och ytterligare analys in vivo.

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Neurovetenskap direkt omvandling hela cell patch Clamp FLEX Vector system CRE-inducible system slice-inspelningar parvalbumin transdifferentiering neurala omvandling
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