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Imaging ad alta risoluzione di dinamiche nucleari in cellule vive sotto tensione uniassiale
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Imaging ad alta risoluzione di dinamiche nucleari in cellule vive sotto tensione uniassiale

Article DOI: 10.3791/59474-v • 09:20 min • June 2nd, 2019
June 2nd, 2019 •
1, 1,2, 1,2,3
1BioSystems and Micromechanics Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, CREATE, 2Department of Material Science and Engineering, Massachusetts Institute of Technology, 3Department of Biological Engineering, Massachusetts Institute of Technology

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Utilizzando un dispositivo progettato in precedenza per applicare la deformazione meccanica alle celle aderenti, questo documento descrive una geometria del substrato ridisegnata e un apparato personalizzato per l'imaging a cella singola ad alta risoluzione delle cellule tese con un obiettivo di immersione in olio 100x.

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Bioingegneria problema 148 stiramento delle cellule deformazione a trazione meccanobiologia della differenziazione cellule progenitrici dell'oligodendrocyte dinamiche nucleari Meccanotrasduzione
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