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Avbildning Kalciumdynamik i subpopulationer av mus bukspottskörteln ö-celler
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Avbildning Kalciumdynamik i subpopulationer av mus bukspottskörteln ö-celler

Article DOI: 10.3791/59491-v • 08:03 min • November 26th, 2019
November 26th, 2019 •
1,2, 1, 3, 1,4,5
1Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, 2Lund University Diabetes Centre, Unit of Molecular Metabolism, Clinical Research Centre, Malmö University HospitalMalmö, 3Institute of Neuroscience of Physiology, Department of Physiology, Metabolic Research Unit, University of Göteborg, 4Oxford National Institute for Health Research, Biomedical Research Centre, 5Life and Medical Sciences, University of Hertfordshire

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Här presenterar vi ett protokoll för avbildning och kvantifiering av kalciumdynamik i heterogena cellpopulationer, till exempel pankreasceller. Fluorescerande reportrar levereras i det perifera lagret av celler inom Holmen, som sedan immobiliseras och avbildas, och per cell analys av dynamiken i fluorescensintensiteten utförs.

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Biologi kalciumdynamik Time-lapse Imaging Langerhanska öar α-celler laserscanning konfokalmikroskopi tidsserie signalbehandling
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